The Strand-seq Core at the BC Cancer Research Institute offers production of Strand-seq data on a fee-for-service basis using state of the art instrumentation and innovative protocols. Experienced Core staff support the needs of researchers with tailored Strand-seq workflows including culturing primary cells or cell lines, constructing single-cell libraries using the cellenONE nanolitre liquid handler and cell isolation system, and next-generation short read DNA sequencing with the AVITI. Inquiries about available Strand-seq services, custom technical Strand-seq needs, and pricing are welcome.
The single cell Strand-seq technology was developed in the Lansdorp lab (1) and has proven to be a key technology to advance a wide variety of studies. Strand-seq has been used for de novo genome assembly and mapping genomic structural variants for genome instability and mutation studies in Nature (2,3), Cell (4) and Science (5). It was recently shown that the unique chromosome-length haplotype information produced by Strand-seq (6) can be leveraged using long-read methylation data at imprint locations to assign any allele on any chromosome in a person to its parent of origin without analysis of parental DNA (7).
Strand-seq library production has been improved by constructing libraries in nanoliter volumes in open nanoliter arrays (8). This advance has resulted in a major increase in the quality of Strand-seq libraries and throughput of library construction.
