The BC Cancer Integrated Mouse Modelling Services (IMMS) was initiated in 2020 as an expansion of the Center for Genetic Modeling (CGM) and Terry Fox Laboratory Transgenics Core after over a decade of successful operations. Our experienced staff supports a vibrant and diverse research community, guaranteeing the necessary support to conduct biomedical research and answer essential questions. The IMMS is situated within the BC Cancer Research Institute (BCCRI), which houses a 1,995 m2 rodent facility (ARC) that provides a barrier environment matching the highest standards of pathogen control for commercial facilities around the world. A biohazard Containment Level III facility, an Embryonic Stem (ES) cell lab, and mouse phenotyping suites also exist as infrastructure support for the IMMS.

Center for Genetic ModelingThe IMMS has state of the art capabilities for generating mouse mutant models through ES cell gene targeting and pronuclear microinjection of mouse zygotes.  In addition the IMMS provides services for cryo-preservation and mouse re-derivation, and has an ES cell core that can derive and manipulate various strains of ES cells.  The CGM also has a mandate to develop or bring in new platform technologies to make the generation of mouse mutants quicker and easier as well as to develop technologies to further facilitate mouse embryo manipulations.

The IMMS can provide the following on a fee-for-service basis:

  • Pronuclear injections into mouse zygotes
  • Blastocyst injections with any strain of ES cells
  • Gene targeting into ES cells
  • CRISPR/Cas9 gene editing in ES cells, zygotes
  • Embryonic Stem Cell (ESC) derivation
  • ESC culture
  • Mouse Embryonic Fibroblast production (MEF)
  • Cre- and Flp-mediated recombination
  • Mouse strain rederivation (fresh or frozen embryos)
  • in-vitro fertilization (fresh or frozen sperm)
  • Cryopreservation of mouse strains (sperm or embryo)
  • Embryo harvest at various stages of development
  • Timed matings
  • Superovulation
  • Surgical services

Send an Inquiry

Assistant Manager: Nicole Hofs
E. nhofs@bccrc.ca
T: 604-675-8000 ext. 7759
 

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